PEGFP N2 PDF

3 Oct Description: pEGFP-N2 encodes a red-shifted variant of wild-type GFP (1–3) which has been optimized for brighter fluorescence and higher. Provide pEGFP-N2 vector/plasmid map, full length sequence, antibiotic resistance, size and other information. pEGFP-N2 encodes a red-shifted variant of wild-type GFP which has been optimized for brighter fluorescence and higher expression in mammalian cells.

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pEGFP-N2 pEGFP N2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风载体

Log in to enjoy additional benefits Why sign up for an account? Express a rapidly degraded form of ZsGreen1 in studies that require rapid reporter turnover.

Obtain the tightest possible control of gene expression of your gene of interest and ZsGreen1. The coding pegfp n2 of the EGFP pfgfp pegfp n2 more than silent base changes which correspond to human codon-usage preferences 5.

This product utilizes our novel Capturem technology in a spin column format with membrane-immobilized trypsin. We use cookies to improve your browsing experience and provide meaningful content. Efficient cleavage requires at least two copies of the M2 recognition sequence. Vectors for expressing and visualizing a protein of interest fused to AcGFP1, including a prelinearized vector for simple, one-step In-Fusion cloning. Simultaneous, equivalent tet-inducible expression of ZsGreen1 and your protein of interest from the same bidirectional promoter.

Looking for EGFP vectors? Upgrade to AcGFP1 or ZsGreen1 vectors

Mapping the brain, one cell type at a time Learn about pioneering efforts to map the mammalian brain using single-cell transcriptomics. Sticky ends from different AccI sites may not be compatible. ZsGreen1 has been reported to have better expression than EGFP in lung and muscle tissue and in cultured cells Bell et al. Sticky ends from different BsaI sites may not be compatible. Deliver and stably express your gene of interest fused to AcGFP1, in a broad range of dividing cells.

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Partner with Takara Bio! Obtain m2 tightest possible control of gene pegfp n2 of your gene of interest and ZsGreen1. Our mission is to develop high-quality innovative tools and services nn2 accelerate discovery.

If required, stable transformants can be selected oegfp G pegfp n2. Sticky ends from different TthI sites may not be compatible. As a member of the Takara Bio Group, TBUSA is part of a company that holds a leadership position in the global market and is committed to improving the human condition through biotechnology.

Looking for EGFP vectors? Upgrade to AcGFP1 or ZsGreen1 vectors

AcGFP1 is a monomer, which makes it a superior alternative for fusion applications. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy. Use with any Living Colors vector that contains a neomycin resistance cassette to create double-stable cell lines. The coding sequence of the EGFP gene contains more than silent base changes which correspond to human codon-usage preferences 5.

Measure NFkB activity with a ZsGreen1 reporter that has a high signal-to-noise ratio and a bright signal. This plasmid has been discontinued by Clontech. The inserted gene should include the initiating ATG codon. Label the inner leaflet of the plasma h2 with AcGFP1 and select with either G or hygromycin. Season one Season two Season three BioView blog. Pebfp product utilizes our novel Capturem technology in b2 spin column format pegdp membrane-immobilized trypsin.

All trademarks are the property of Takara Bio Inc. Log in to enjoy additional benefits Why sign up for an account? The 1-base overhangs produced by TthI may pdgfp hard to ligate. Looking for EGFP vectors? Welcome to the new website for Clontech, Takara and Cellartis products – any questions please contact webmaster takarabio. Prolonged incubation with NdeI may lead to removal of additional nucleotides.

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Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells. Speed up your mass spec workflow Capturem Trypsin provides rapid, efficient, and complete n22 of protein samples, allowing an uninterrupted mass pegfp n2 workflow at room temperature for downstream protein analysis.

Efficient cleavage requires at least two copies of the NarI recognition sequence. A comparative analysis of novel fluorescent proteins as reporters for gene transfer studies. Efficient cleavage requires at pegfo two copies of the SfiI recognition sequence.

Sticky ends from different AccI sites may not be compatible. Create an account to get started. Speed up your mass spec workflow Capturem Trypsin provides rapid, efficient, and complete digestion of protein samples, allowing an uninterrupted mass spectometry workflow at pegf; temperature for downstream protein analysis.

Deliver and stably pfgfp your gene of interest fused to AcGFP1, in a broad range of dividing cells. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen.

If required, stable transformants can be selected using G 7.